DNA Fingerprint analysis is based on the "Southern" hybridization technique. In this method:

A.	Non-radioactive cellular RNA, separated by electrophoresis and blotted onto a membrane filter, is tested for hybridization with a radioactive gene-specific probe of either RNA or DNA.
B.	Radioactive DNA restriction fragments, separated by electrophoresis and blotted onto a membrane filter are tested for hybridization with a non-radioactive, gene-specific probe of either RNA or DNA.
C.	Radioactive cellular RNA, separated by electrophoresis and blotted onto a membrane filter is tested for hybridization with a radioactive, gene-specific probe of either RNA or DNA.
D.	DNA research is done south of the equator.
E.	Non-radioactive DNA restriction fragments, separated by electrophoresis and blotted onto a membrane filter are tested for hybridization with a radioactive, gene-specific probe of either RNA or DNA.

The Biology Project
University of Arizona
Tuesday, August 20, 1996
hallick@u.arizona.edu

http://www.biology.arizona.edu
All contents copyright © 1996. All rights reserved.